Study of Real-Time PCR Assays for Rapid Detection of Food-Borne Pathogens
نویسندگان
چکیده
منابع مشابه
Improved template preparation for PCR-based assays for detection of food-borne bacterial pathogens.
Shigella flexneri, Salmonella enterica serotype Typhimurium, and Listeria monocytogenes were applied to FTA filters, and the filters were used directly as templates to demonstrate their sensitivity and applicability in PCR-based detection assays. With pure cultures, the sensitivities of detection by FTA filter-based PCR were 30 to 50 and 200 CFU for the gram-negative enterics and Listeria, resp...
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The current study is focused on determining the mRNA expression levels of tetranectin, to detect oral squamous cell carcinoma (OSCC) and thus aiding in its classification at an early stage. RNA was isolated and cDNA synthesis was performed from the saliva samples of the patients and healthy individuals. A semiquantitative PCR based analysis was performed prior to quantitative and expression bas...
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Cereulide-producing Bacillus cereus can cause an emetic type of food-borne disease that mimics the symptoms provoked by Staphylococcus aureus. Based on the recently discovered genetic background for cereulide formation, a novel 5' nuclease (TaqMan) real-time PCR assay was developed to provide a rapid and sensitive method for the specific detection of emetic B. cereus in food. The TaqMan assay i...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 2005
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.79.644